A new microscopy approach developed by the Vaziri Lab at Rockefeller University takes the next steps for high-speed, volumetric recording of neuroactivity in the mouse cortex. The developed so called Light Beads Microscopy (LBM) is a mesoscopic imaging approach able to record up to 1 million neurons within 5.4×6×0.5 mm^3 volumes at 2 Hz frame rate.
Hence, the technology can enable neuroscientists to understand cortex-wide information processing and ultimately how the brain works.
Measurements have been performed with the White Dwarf OPCPA for 3-photon microscopy.
Reference: Demas, J., Manley, J., Tejera, F. et al. High-speed, cortex-wide volumetric recording of neuroactivity at cellular resolution using light beads microscopy. Nat Methods (2021). https://doi.org/10.1038/s41592-021-01239-8
Rapp OptoElectronic and Class 5 Photonics make the next step in commercial systems enabling
three-photon microscopy for deep brain imaging. Joint measurements have been
supported by the renowned University Medical Center Hamburg-Eppendorf (UKE) and show
promising results in comparison to standard two-photon microscopy.
Two-photon image (2p) and three-photon image (3p) of the dentate gyrus region in the hippocampus of a mouse. Depth 500 μm. Measurements are the result of a joint campaign of Rapp OptoElectronic and Class 5 Photonics in Hamburg with sample preparation provided by the University Medical Center Hamburg-Eppendorf (UKE).
Another White Dwarf for 3-photon microscopy was installed in a bio-imaging lab in Germany. With > 5 W and 40 fs at 1300 nm the WD-1300 provides the highest average power and peak intensity on the market for advanced microscopy applications.
Additionally, our one-box solution and robust engineering achieves excellent power stability and pulse-to-pulse stability (typically < 0.5 %). The integrated, industrial Coherent Monaco 60 W pump laser provides additional reliability and user friendly operation of the White Dwarf system.
Hence, biologists can focus on their in-vivo experiments while keeping laser maintenance to a minimum and increasing up-time.
The installed system is upgradable with a second channel at 1700 nm (WD-1300-dual). An independent, second OPCPA channel is optimized at 1700 nm and hence multi-color experiments are possible when running the system in a parallel mode (1300 and 1700 nm simultaneously). Alternatively, the user can switch between 1300 and 1700 nm to double the average power at each wavelength for deep brain imaging.
Our CTO Michael presented at Photonics West 2021 our high-power OPCPA system for in vivo multi-photon microscopy: White Dwarf OPCPA.
The White Dwarf OPCPA is a compact and robust laser system especially designed for multi-photon microscopy at high performance parameters.
Publication: High power OPCPA system for in-vivo 2- and 3-photon brain imaging, Proc. SPIE 11648, Multiphoton Microscopy in the Biomedical Sciences XXI, 1164819 (5 March 2021); doi: 10.1117/12.2578713
Additionally, the White Dwarf OPCPA can be configured for three-photon excitation of red fluorescent proteins at 1700 nm. Pulse energies of more than 2 µJ at 1 MHz repetition rate allow fast imaging of deep-tissue.
The White Dwarf OPCPA implements the Coherent Monaco industrial fiber laser as pump source for long-term stability, reliability and turn-key operation.