High-resolution deep brain imaging up to a depth of 1.4 mm using White Dwarf OPCPA

A new microscopy technique developed at EMBL in Heidelberg enables highest imaging resolution for in vivo neuronal activity measurements deep in the brain, driven by a Class 5 Photonics White Dwarf OPCPA. Indirect adaptive optics (AO) in combination with three-photon microscopy reaches near-diffraction-limited resolution of very fine structures in the hippocampus up to a depth of 1.4 mm.

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Fine dendritic processes in the hippocampus can be resolved with the newly developed microscope at EMBL Heidelberg: Scale bar is 20 μm on left pictures showing a depth of 1.243 to 1.423 mm on yz plot; on zoomed region scale bar is 2 µm showing the cross-section of a dendrite.
Streich, L., Boffi, J.C., Wang, L. et al. High-resolution structural and functional deep brain imaging using adaptive optics three-photon microscopy. Nat Methods 18, 1253–1258 (2021). https://doi.org/10.1038/s41592-021-01257-6