White Dwarf OPCPA

WHITE DWARF OPCPA 5 W POWERED BY COHERENT

for 3-photon microscopy

The White Dwarf is a compact and a robust laser system specially designed for 3-photon microscopy, offering now simultaneously 1300 and 1700 nm at outstanding performance parameters. With the highest peak power available for 3-photon microscopy on the market, the White Dwarf allows neuroscientists to image deeper, faster
and at better resolution than ever before at green and red fluorescent markers.

> 5 µJ at 1300 nm @ 1 MHz
> 5 µJ at 1700 nm @ 1 MHz
repetition rates up to 10 MHz available
short pulse durations < 50 fs at sample
pumped by Coherent Monaco industrial femtosecond laser

Try the White Dwarf in your lab!

Applications
Specifications
Performance
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Bio imaging

Volumetric imaging at high resolution / larger imaging volume and higher frame rates by higher repetition rate / deep tissue penetration in all spectral windows / highest multiphoton signal and better signal-to-noise ratio with shorter pulses at lower heat load

• In vivo brain imaging

• Multiphoton absorption imaging

• Deep optical imaging
of tissue

Volumetric imaging of deep brain tissue

The high average power of the White Dwarf OPCPA allows imaging of brain tissue - beyond 1 mm - in a large volume while maintaining single-cell resolution.

3D rendering of Hybrid Multiplexed Sculpted Light Microscopy recording in mouse PPC, cytosolic GCaMP6f (green: 2p, cyan: 3p), Vaziri Lab.
(Weisenburger et al., Volumetric Ca2+ Imaging in the Mouse Brain Using Hybrid Multiplexed Sculpted Light Microscopy, Cell (2019), https://doi.org/10.1016/j.cell.2019.03.011).

Near-diffraction-limited imaging of deep brain tissue

Adaptive Optics (AO) enables high-resolution 3P deep brain imaging at depths down to 1.4 mm in the hippocampus of mouse brain.
(Streich, L., Boffi, J.C., Wang, L. et al. High-resolution structural and functional deep brain imaging using adaptive optics three-photon microscopy. Nat Methods 18, 1253–1258 (2021). https://doi.org/10.1038/s41592-021-01257-6)

Our White Dwarf OPCPA powered by Coherent has its pump laser already on board to enter the next level of stability. We integrate the Coherent Monaco industrial femtosecond laser on a small footprint with state-of-the-art White Dwarf OPCPA technology to bring industrial performance to scientific output parameters.

This White Dwarf version is especially designed for multi-photon microscopy at high performance parameters. The highest peak power available for three-photon microscopy on the market allows neuroscientists to image deeper and at higher speed.

	WD-960	WD-1300	WD-1700	WD-1300-dual
Central wavelength	960 nm	1300 nm	1700 nm	Two synchronized outputs: 960 and 1300 nm, or 1300 and 1700 nm
Pulse duration (FWHM)	< 50 fs	< 50 fs	< 65 fs	< 50 fs at 1300 nm, < 65 fs at 1700 nm
Average power	> 5 W	> 5 W	> 5 W	> ca 2.5 W each output
Pulse energy	> 5 µJ	> 5 µJ	> 5 µJ	> ca 2.5 µJ each output
Repetition rate	single-shot to 10 MHz	single-shot to 10 MHz	single-shot to 4 MHz	single-shot to 4 MHz
Beam quality M2	< 1.3	< 1.3	< 1.3	< 1.3
Power stability (12 hrs)	< 1 %	< 1 %	< 1 %	< 1 %
Dimensions	80 cm x 80 cm	80 cm x 80 cm	80 cm x 120 cm	80 cm x 120 cm
Pump laser incl.	Coherent Monaco 60 W	Coherent Monaco 60 W	Coherent Monaco 60 W	Coherent Monaco 60 W

Example sytem White Dwarf with integrated Coherent Monaco.

Spectrum of compressed WD-1300 at 2 MHz, 3 µJ, 40 fs pumped by Coherent Monaco 1035-80-60. Additionally, three photon action cross section spectra of GCaMP-6-f is shown (taken from https :://www janelia org/lab/harris lab/research/photophysics/two photonfluorescent probes).

Short-term pulse-to-pulse stability measured with fast photodiode and oscilloscope over 0.8 ms: Pump laser Coherent Monaco 60 W, 2 MHz repetition rate, White Dwarf OPCPA WD-1300, average pulse energy > 2 µJ (4 W) and 0.4 % standard deviation.

Spectrum of compressed WD-1300-dual at 1700 nm at 4 MHz, 1.5 µJ, < 70 fs pumped by Coherent Monaco 1035-80-60.

Long-term power stability measured with powermeter (averaged shots) over more than 2 days: Pump laser Coherent Monaco 60 W, 2 MHz repetition rate, White Dwarf OPCPA WD-1300, 4.21 W average power and 0.39 % standard deviation.

Data sheet

Applications: 3-photon microscopy

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