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White Dwarf laser for two photon absorption nanolithography and multiphoton microscopy
  • White Dwarf laser for two photon absorption nanolithography and multiphoton microscopy

White Dwarf dual – dual channel lasers for multiphoton microscopy

Dual Channel Lasers for 2- and 3-photon volumetric- and deep-tissue imaging with red and green markers
  • wavelength: 920 nm , 960 nm, 1300 nm and 1700 nm
  • average power: > 3 W or > 5 W
  • optimized for multicolor volumetric and deep imaging
  • based on industrial Yb-fiber femtosecond laser
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Introduction

High performance femtosecond laser for multiphoton microscopy (2p and 3p microscopy)

The White Dwarf is a femtosecond laser system specially designed for two and three-photon microscopy offering highest performance and robustness.

The White Dwarf is a compact and robust femtosecond laser system specially designed for 3-photon microscopy, offering now simultaneously 1300 and 1700 nm at outstanding performance parameters. With the highest peak power available for 3-photon microscopy, the White Dwarf allows neuroscientists to image deeper, faster and at better resolution than ever before at green and red fluorescent markers.

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Features

Optimized for multi-color imaging

Two simultaneous outputs at 1300 nm and 1700 nm or 960 nm or 920 nm

Ideal for deep imaging

> 5 W average power after compressor

Proven in the field

Trusted by 20+ neuroscience labs worldwide

Reliability

Robust design and one-box system

1035 nm output

Output for optogenetics optionally available

Specs

Specifications WD-1300S/1300/1300-pro WD-1300-1700-S WD-1300-1700 WD-1300-1700-pro
Switchable 3p Outputs — Output #1 Output #2 Output #1 Output #2 Output #1 Output #2
Central wavelength 1300 nm 1300 nm 1700 nm 1300 nm 1700 nm 1300 nm 1700 nm
Tuning range (nm) — 1250 – 1350 nm 1650 – 1750 nm 1250 – 1350 nm 1650 – 1750 nm 1250 – 1350 nm 1650 – 1750 nm
Avg. Power 1.5 W / 3 W / 5 W > 1.5 W > 1 W > 3 W > 2 W > 4.5 W > 3 W
Pulse duration FWHM < 50 fs < 50 fs < 65 fs < 50 fs < 65 fs < 50 fs < 65 fs
Dispersion Compensation up to -12,000 fs² up to -12,000 fs² up to -12,000 fs² up to -12,000 fs² up to -12,000 fs² up to -12,000 fs² up to -12,000 fs²
Rep rate Up to 5 MHz 1 MHz 1 MHz up to 2 MHz up to 2 MHz up to 2 MHz up to 2 MHz
Pulse energy (@ 1 MHz) 1.5 µJ / 3 µJ / 5 µJ > 1.5 µJ > 1 µJ > 3 µJ > 2 µJ > 4.5 µJ > 3 µJ

Performance

Spectrum
Spectrum of 1300 nm for 3-photon microscopy with GFP. Additionally, three photon action cross section spectra of GCaMP-6-f is shown (taken from https :://www janelia org/lab/harris lab/research/photophysics/two photonfluorescent probes).
Stability
Long-term power stability measured with powermeter (averaged shots) over more than 2 days.

Applications

  • Multiphoton microscopy
  • Volumetric brain imaging
  • Multi-color imaging
  • Adaptive optics (AO) three-photon microscopy

Downloads

FAQ

Publications

  • December 21, 2023
    Amr Tamimi, Martin Caldarola,…, Robert Prevedel, Deep mouse brain two-photon near-infrared fluorescence imaging using a superconducting nanowire single-photon detector array
    https://doi.org/10.48550/arXiv.2312.14042
  • September 30, 2021
    Streich, L., Boffi, J.C., Wang, L. et al. High-resolution structural and functional deep brain imaging using adaptive optics three-photon microscopy. Nat Methods 18, 1253–1258 (2021).
    https://doi.org/10.1038/s41592-021-01257-6
  • May 2, 2019
    Weisenburger et al., Volumetric Ca2+ Imaging in the Mouse Brain Using Hybrid Multiplexed Sculpted Light Microscopy, Cell (2019)
    https://doi.org/10.1016/j.cell.2019.03.011

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