White Dwarf hybrid – lasers for simultaneous 2-photon and 3-photon microscopy

Laser for simultaneous 2-photon and 3-photon imaging

wavelength: 960 nm and 1300 nm
average power: > 3 W or > 5 W
optimized for hybrid microscopy
based on industrial Yb femtosecond laser

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Details

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Introduction

High performance laser for two-photon and three-photon microscopy

The White Dwarf is a laser system specially designed for multi-photon microscopy offering highest performance and robustness.

The White Dwarf is a compact and robust laser system specially designed for multi-photon microscopy, offering now simultaneously 960 and 1300 nm at outstanding performance parameters. With the highest peak power available for hybrid multi-photon microscopy, the White Dwarf allows neuroscientists to image deeper, faster and at better resolution than ever before.

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Features

Optimized for hybrid microscopy (2p/3p)

Two simultaneous outputs at 960 nm and 1300 nm

Ideal for deep imaging

> 5 W average power after compressor

Proven in the field

Trusted by 20+ neuroscience labs worldwide

Reliability

Robust one-box design

1035 nm output

Output for optogenetics optionally available

Specs

	WD-1300-960	WD-1300-960-pro
Central wavelength	960 nm and 1300 nm (switchable or simultaneous)	960 nm and 1300 nm (switchable or simultaneous)
Average power	> 3 W	> 5 W
Pulse duration FWHM	< 100 fs at 960 nm and < 50 fs at 1300 nm	< 100 fs at 960 nm and < 50 fs at 1300 nm
Dispersion compensation optional	up to -20.000 fs^2 at 960 nm and up to -12.000 fs^2 at 1300 nm	up to -20.000 fs^2 at 960 nm and up to -12.000 fs^2 at 1300 nm
Pulse energy compressed	> 3 µJ at 1 MHz	> 5 µJ at 1 MHz
Repetition rate	up to 5 MHz	up to 5 MHz
Beam quality M2	< 1.3	< 1.3
Power stability 12 hrs	< 1 %	< 1 %
Dimensions	80 cm x 120 cm	80 cm x 120 cm

Performance

Applications

Multiphoton microscopy
Volumetric brain imaging
Hybrid microscopy (HyMS)

Downloads

FAQ

Publications

May 2, 2019

Weisenburger et al., Volumetric Ca2+ Imaging in the Mouse Brain Using Hybrid Multiplexed Sculpted Light Microscopy, Cell (2019)

https://doi.org/10.1016/j.cell.2019.03.011
September 30, 2021

Streich, L., Boffi, J.C., Wang, L. et al. High-resolution structural and functional deep brain imaging using adaptive optics three-photon microscopy. Nat Methods 18, 1253–1258 (2021).

https://doi.org/10.1038/s41592-021-01257-6
March 6, 2024

Jason Manley, Jeffrey Demas, Hyewon Kim, Francisca Martínez Traub, Alipasha Vaziri, Simultaneous, cortex-wide dynamics of up to 1 million neurons reveal unbounded scaling of dimensionality with neuron number, Neuron 2024

https://doi.org/10.1016/j.neuron.2024.02.011
December 21, 2023

Che-Hang Yu, Yiyi Yu,…, Alipasha Vaziri,…, David Fitzpatrick,…, Prakash Kara,…, Ikuko T. Smith & Spencer LaVere Smith, The Cousa objective: a long-working distance air objective for multiphoton imaging in vivo, Nature Methods 2023

https://doi.org/10.1038/s41592-023-02098-1
May 15, 2024

Bellafard, A., Namvar, G., Kao, J.C. et al. Volatile working memory representations crystallize with practice. Nature 629, 1109–1117 (2024)

https://doi.org/10.1038/s41586-024-07425-w

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