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White Dwarf 3p – three photon microscopy laser

Laser for 3-photon deep-tissue imaging at highest frame-rates
  • laser wavelength: 1300 nm and 1700 nm
  • repetition rate: up to 5 MHz
  • average power: up to 5 W
  • high-speed or high-resolution deep imaging
  • based on industrial Yb femtosecond laser

Introduction

High performance femtosecond laser for three-photon microscopy (3p microscopy)

The White Dwarf 3p is a femtosecond laser system specially designed for three-photon microscopy offering highest performance and robustness.

With the highest peak power available for 3-photon microscopy, the White Dwarf allows neuroscientists to discover new frontiers in deep brain imaging. Additionally, the one-box solution offers compactness and robustness.

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More Information

Features

High power for fast and deep imaging

> 5 W average power after compressor

Proven in the field

Trusted by 30+ neuroscience labs worldwide

Reliability

Robust one-box design

1035 nm output

Output for optogenetics optionally available

Specs

Specifications WD-1300S/1300/1300-pro WD-1300-1700 WD-1300-1700-pro WD-1300-1700-ultra
Switchable 3p Outputs (Central wavelength) 1300 nm 1300 nm 1700 nm 1300 nm 1700 nm 1300 nm 1700 nm
Tuning range (nm) 1250 – 1350 nm 1650 – 1750 nm 1250 – 1350 nm 1650 – 1750 nm 1250 – 1350 nm 1650 – 1750 nm
Avg. Power 1.5 W / 3 W / 5 W > 1.5 W > 1 W > 3 W > 2 W > 4.5 W > 3 W
Pulse duration FWHM < 50 fs < 50 fs < 65 fs < 50 fs < 65 fs < 50 fs < 65 fs
Dispersion Compensation up to -12,000 fs² up to -12,000 fs² up to -12,000 fs² up to -12,000 fs² up to -12,000 fs² up to -12,000 fs² up to -12,000 fs²
Rep rate Up to 5 MHz 1 MHz 1 MHz up to 2 MHz up to 2 MHz up to 2 MHz up to 2 MHz
Pulse energy (@ 1 MHz) 1.5 µJ / 3 µJ / 5 µJ > 1.5 µJ > 1 µJ > 3 µJ > 2 µJ > 4.5 µJ > 3 µJ
AUX Output (1035nm) upon request upon request upon request upon request


Applications

  • Volumetric brain imaging
  • Scanning temporal focusing microscopy (sTeFo)
  • Adaptive optics (AO)


FAQ


Publications

  • December 21, 2023
    Che-Hang Yu, Yiyi Yu,…, Alipasha Vaziri,…, David Fitzpatrick,…, Prakash Kara,…, Ikuko T. Smith & Spencer LaVere Smith, The Cousa objective: a long-working distance air objective for multiphoton imaging in vivo, Nature Methods 2023
  • June 21, 2023
    Marc Cicero Schubert, Stella Judith Soyka et. al., Deep intravital brain tumor imaging enabled by tailored three-photon microscopy and analysis
  • November 28, 2022
    Klioutchnikov, A., Wallace, D.J., Sawinski, J. et al., A three-photon head-mounted microscope for imaging all layers of visual cortex in freely moving mice, Nat Methods (2022).
  • September 30, 2021
    Streich, L., Boffi, J.C., Wang, L. et al. High-resolution structural and functional deep brain imaging using adaptive optics three-photon microscopy. Nat Methods 18, 1253–1258 (2021).
  • May 2, 2019
    Weisenburger et al., Volumetric Ca2+ Imaging in the Mouse Brain Using Hybrid Multiplexed Sculpted Light Microscopy, Cell (2019)